Microarray analysis of cell cycle and apoptosis biomarkers (p16, p21, p27, p53, Bcl-2, Bax, Bcl-xL and Cyclin D1) in thyroid papillary carcinoma, papillary microcarcinoma and lymph node metastasis of thyroid papillary carcinoma

Akkaş Akgün G., Temiz P., Ayhan S., Uğur Duman F., Aydede H.

Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, vol.8, no.3, pp.419-425, 2021 (Peer-Reviewed Journal)


Objective: The aim of this study was to identify the role of apoptosis and cell cycle associated gene products in the pathogenesis of thyroid papillary carcinoma (TPC) and its lymph node metastasis. Materials and Methods: Cases of thyroid papillary carcinoma (n=35), thyroid papillary microcarcinoma (TPMC) (n=22), TPC-lymph node metastasis (TPC-LNM) (n=12), and adenomatous nodule (AN) (n=20) were examined using tissue microarray method (TMA) by immunohistochemistry staining for p16, p21, p27, p53, bcl-2, bax, bcl-xL and cyclin D1. Results: Bcl-2 staining of the ANs was significantly differed from those of malignant groups. p53, p16, p21 staining percentages were significantly higher in the malignant groups than in the benign lesions. TPC-LNM group had higher p16 and cyclin D1 positivity than the primary tumor groups. The most remarkable difference of p27 staining was between the TPC-LNM and TPC groups. Conclusion: We concluded that cell cycle regulators, especially bcl-2 family, play important roles in TPC carcinogenesis. The cyclin-dependent kinase inhibitors acting on the cyclin-CDK complex (p16, p21, p27) were more associated with potential for malignancy, progression and poor prognosis. p53 plays an important role in the TPC pathogenesis by interacting with the proteins regulating both apoptosis and the cell cycle.