Rapid Fibroblast Cell Culture Characterization withImpedimetric Label-free Heat Shock Protein – 47Biosensor


Harmancı D., Uygun Z. O. , Koçak Sezgin A. , Demirdöver C., Girgin Sağın F., Akdoğan G.

Electroanalysis, cilt.32, ss.1-7, 2020 (SCI Expanded İndekslerine Giren Dergi)

  • Cilt numarası: 32
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/elan.202060264
  • Dergi Adı: Electroanalysis
  • Sayfa Sayıları: ss.1-7

Özet

Abstract: In this study, for the first time in literature, an
impedimetric biosensor technology was developed to
determine Heat Shock Protein-47 (HSP-47) for the
characterization of primary skin fibroblast cells. Gold
nanoparticle electrodes (GNPE) were modified with
amino-functionalized graphene oxide layers and anti-
HSP-47 were immobilized on this layer. The performance
of the biosensor was tested with real samples as cell
lysates. The low detection limit (LOD) and the linear
detection limit (LOQ) of the biosensor were calculated as
9.47 pg/mL and 3.23 pg/mL, respectively. Linear measure-
ment range was obtained between 10–160 pg/mL HSP-47.
The reproducibility of the biosensor was good.