LoxBlock-1 or Curcumin Attenuates Liver, Pancreas and Cardiac Ferroptosis, Oxidative Stress, and Injury in Ischemia/Reperfusion-Damaged Rats by Facilitating ACSL/GPx4 Signaling

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Kar F., Yıldız F., Kar E., Hacıoğlu C., Burukoğlu Dönmez D., Şentürk H., ...More

TISSUE AND CELL, vol.82, no.102114, pp.1-10, 2023 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 82 Issue: 102114
  • Publication Date: 2023
  • Doi Number: 10.1016/j.tice.2023.102114
  • Journal Name: TISSUE AND CELL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1-10
  • Kütahya Health Sciences University Affiliated: Yes

Abstract

In this study, the effects of the pretreatment of Curcumin and LoxBlock-1 on liver, pancreas,

and cardiac dysfunction following Ischemia-Reperfusion-induced (IR) Acute Kidney Injury (AKI)

were investigated through the mechanisms of oxidative stress and ferroptosis. Total antioxidant status

(TAS), total oxidant status (TOS) and oxidative stress index (OSI) parameters in the tissue were

analyzed to investigate the oxidative stress occurring in the liver, pancreas, and heart, and Acyl-Coa

synthetase long-chain family member (ACSL4). Glutathione peroxidase 4 (GPx4) enzyme levels were

also analyzed by ELISA to investigate the effect on ferroptosis. In addition, hematoxylin-eosin

staining was performed for histopathological examination of the tissues. As a result of biochemical

analyzes, it was observed that oxidative stress parameters increased significantly in the IR group. In

addition, while the ACSL4 enzyme level increased in the IR group in all tissues, the GPx4 enzyme

level decreased. In the histopathological examination, it was observed that IR caused serious damage

to the heart, liver, and pancreas tissues. The present study shows that Curcumin and LoxBlock-1 have

a protective effect on the liver, pancreas, and cardiac ferroptosis following the effect on AKI. In

addition, Curcumin was found to be more effective than LoxBlock-1 in I/R injury with its antioxidant

property