Does Experimental Morphine Addiction in Rats Change Physiological and Histological Characteristics of the Heart?

Cagliyan H., Görmüş Z. I. S., Solak H., Koca R. Ö., Gültekin B.

Selçuk Tıp Dergisi, vol.38, no.3, pp.128-135, 2022 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 38 Issue: 3
  • Publication Date: 2022
  • Doi Number: 10.30733/std.2022.01558
  • Journal Name: Selçuk Tıp Dergisi
  • Journal Indexes: TR DİZİN (ULAKBİM), Index Copernicus
  • Page Numbers: pp.128-135
  • Kütahya Health Sciences University Affiliated: Yes


Aim: Morphine is one of the most preferred opioids in treatment of chronic pain. Recurrent use can cause addiction. There is no consensus on cardiovascular system treatment/side effects of opioids. In order to investigate effects of opioid addiction on heart, myocardial contractility/histological changes were investigated in rats via experimental morphine addiction/withdrawal. Materials and Methods: 32 adult male Wistar albino rats used for study, which was officially completed on 28-05-2021, were divided into Control(C), Morphine(M), Morphine+Naloxone(MN) groups randomly. In GroupC 10mg/kg 0.9% NaCl solution, in GroupM-MN 10mg/kg morphine were administered subcutaneously for 7 days. After the last administration of morphine, 3mg/kg NaCl was given to GroupC-M, 3mg/kg naloxone was given to GroupMN intraperitoneally. Signs of morphine withdrawal were observed for 30 minutes and scored. 3-4mm strips of atria were hung in isolated organ bath chambers. Tension was adjusted to 2g. Adrenaline-induced contractions (0.001M) were observed. Changes in tension were recorded. SAS University Edition 9.4 program was used for statistical analysis. Results: Morphine withdrawal behaviours were observed in GroupMN. There was no statistically significant difference between atrial contractility tension values of GroupC-M-MN(p>0.05). Pre-adrenaline tension values were higher in GroupM-MN than in GroupC. But the greatest contraction increase between 15minutes before/after adrenaline-induced data was in GroupC. Conclusion: Morphine addiction/withdrawal didn’t cause inotropic/chronotropic changes. No histological differences were observed in mast cells. These results may constitute a positive resource for the heart for systems analysis in morphine addicts.