TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.43, no.5, pp.98-99, 2019 (Journal Indexed in SCI Expanded)
OBJECTIVES:The aim of this study was to investigate the effect of
curcumin(10μM) on neurotoxicity and oxidative stress caused by the
application of sodium fluoride(10mM) and mercuric chloride(5μM) on rat brain
synaptosomes obtained in vitro to prevent degenerative damage on synaptosomes.
MATERIALS and METHODS:In our study, sinapotosomes were prepared
using brain obtained from 5 Wistar Albino rats. A Control, B 10 mM Sodium
Fluoride, C 5 μM Mercury Chloride, D 5 μM Mercury Chloride+10 μM
Curcumin, E 10 mM Sodium Fluoride+10 μM Curcumin, F 10 mM Sodium
Fluoride+5 μM Mercury Chloride+10 μM Curcumin six groups were formed.
DNA fragmation, Adenosine Deaminase(ADA), Malondialdehyde(MDA)
and Superoxide Dismutase(SOD) levels were measured on the synaptosomes.
RESULTS:MDA and DNA fragmentation levels significant increased,
SOD and ADA levels decreased in the B and C groups compared to the
A group(p<0.01). In curcumin-treated groups D and E, MDA and DNA
fragmentation levels decreased, SOD and ADA levels increased statistically
when compared with A, B and C groups(p<0.01). The levels of MDA and
DNA fragmentation in the F group were statistically decreased compared
to A, B and C groups, whereas levels of SOD and ADA statistically
increased(p<0.01). There was no statistically difference in the levels of MDA,
SOD, ADA and DNA fragments in group F compared to group D and E(p> 0.01).
CONCLUSIONS:In our study, it was observed that administration of
sodium fluoride and mercury chloride increased oxidative stress and
neurotoxicity and damaged the antioxidant system. It has been observed
that Curcumin may reduce increased oxidative stress and neurotoxicity
Keywords: Brain, Neurotoxicity, Free Radicals, Antioxidant