THE EFFECT OF CURCUMIN AGAINST FLUORIDE AND MERCURY INDUCED NEURODEGENERATION AND OXIDATIVE INJURY


Kar F. , Hacıoğlu C., Kar E., Kara Y., Uslu S.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.43, no.5, pp.98-99, 2019 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Abstract
  • Volume: 43 Issue: 5
  • Publication Date: 2019
  • Title of Journal : TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Page Numbers: pp.98-99

Abstract

OBJECTIVES:The aim of this study was to investigate the effect of

curcumin(10μM) on neurotoxicity and oxidative stress caused by the

application of sodium fluoride(10mM) and mercuric chloride(5μM) on rat brain

synaptosomes obtained in vitro to prevent degenerative damage on synaptosomes.

MATERIALS and METHODS:In our study, sinapotosomes were prepared

using brain obtained from 5 Wistar Albino rats. A Control, B 10 mM Sodium

Fluoride, C 5 μM Mercury Chloride, D 5 μM Mercury Chloride+10 μM

Curcumin, E 10 mM Sodium Fluoride+10 μM Curcumin, F 10 mM Sodium

Fluoride+5 μM Mercury Chloride+10 μM Curcumin six groups were formed.

DNA fragmation, Adenosine Deaminase(ADA), Malondialdehyde(MDA)

and Superoxide Dismutase(SOD) levels were measured on the synaptosomes.

RESULTS:MDA and DNA fragmentation levels significant increased,

SOD and ADA levels decreased in the B and C groups compared to the

A group(p<0.01). In curcumin-treated groups D and E, MDA and DNA

fragmentation levels decreased, SOD and ADA levels increased statistically

when compared with A, B and C groups(p<0.01). The levels of MDA and

DNA fragmentation in the F group were statistically decreased compared

to A, B and C groups, whereas levels of SOD and ADA statistically

increased(p<0.01). There was no statistically difference in the levels of MDA,

SOD, ADA and DNA fragments in group F compared to group D and E(p> 0.01).

CONCLUSIONS:In our study, it was observed that administration of

sodium fluoride and mercury chloride increased oxidative stress and

neurotoxicity and damaged the antioxidant system. It has been observed

that Curcumin may reduce increased oxidative stress and neurotoxicity

Keywords: Brain, Neurotoxicity, Free Radicals, Antioxidant