Research Information System
22nd ESE Biennial Congress, Paris, France, 3 - 06 September 2025, pp.102, (Summary Text)
AIM: The aim of this study was to evaluate the antimicrobial efficacy of NaOCl and NaOCl/HEDP solutions, applied with different temperatures (25°C and 37°C) and irrigation methods, passive ultrasonic activation (PUI) and conventional needle irrigation (CNI), on E. faecalis biofilm in simulated internal root resorption (IRR) cavities. Methodology: Simulated IRR cavities were created in 76 single-rooted maxillary central incisors, which were contaminated with E. faecalis for 21 days to facilitate biofilm formation. The teeth were divided into two main experimental groups according to the applied irrigation solutions (NaOCl or NaOCl/HEDP), as well as a control group. The experimental groups were further divided into four subgroups based on different irrigation solution temperatures (25°C or 37°C) and irrigation methods (PUI or CNI) (n=8). CFU counts were determined for all teeth prior to treatment, followed by root canal preparation. After treatment, CFU counts were measured from the root canal samples, and antimicrobial activity differences between groups were compared using one-way ANOVA and Duncan’s post-hoc test. At 37°C, NaOCl/HEDP was effective regardless of the irrigation method, whereas NaOCl showed greater effectiveness when PUI was applied (p<0.05). Solutions used at 25°C were more effective when PUI was applied for both NaOCl and NaOCl/HEDP (p<0.05). Regardless of irrigation method, NaOCl/HEDP at 37°C was more effective than at 25°C (p<0.05). No difference was observed between NaOCl at 37°C with CNI and at 25°C with PUI (p>0.05). Results: The use of solutions at higher temperatures was found to be more effective in IRR cavities. The use of NaOCl solution at 37°C may be an alternative option to PUI. However, PUI is recommended when solutions are used at 25°C. *This work has been supported by Kutahya Health Sciences University Scientific Research Projects Coordination Unit under grant number TDH-2024-159.