Protective effect of 2-aminoethyl diphenylborinate on acute ischemia-reperfusion injury in the rat kidney


Yildar M., Aksit H., Korkut O., ÖZYİĞİT M. Ö., Sunay B., Seyrek K.

Journal of Surgical Research, vol.187, no.2, pp.683-689, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 187 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1016/j.jss.2013.11.009
  • Journal Name: Journal of Surgical Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.683-689
  • Keywords: 2-APB, Antioxidants, Calcium channels, Kidney failure, Oxidative stress
  • Kütahya Health Sciences University Affiliated: No

Abstract

Background To investigate the protective effect of 2-aminoethyl diphenylborinate (2-APB) against ischemia-reperfusion (I/R) injury in the rat kidney by an experimental study. Materials and methods Thirty male Sprague-Dawley rats were randomly divided into the following three groups: (1) sham group, (2) I/R group, and (3) I/R + 2-APB group. Renal I/R injury was induced by clamping the left renal pedicle for 45 min after right nephrectomy, followed by 3 h of reperfusion. The therapeutic agent 2-APB was administered intravenously at a dose of 2 mg/kg 10 min before renal ischemia. Glutathione, superoxide dismutase, total antioxidant capacity, malondialdehyde, tumor necrosis factor α, interleukin 6, aspartate aminotransferase, alanine aminotransferase, and creatinine levels were measured from blood samples, and the rats were sacrificed subsequently. Tissue samples were scored histopathologically. Visualization of apoptotic cells was performed using the terminal deoxynucleotidyl transferase dUTP nick end labeling staining method. Results 2-APB significantly reduced serum malondialdehyde, tumor necrosis factor α, interleukin 6, aspartate aminotransferase, alanine aminotransferase, and creatinine levels in the I/R injury group. However, glutathione, superoxide dismutase, and total antioxidant capacity levels increased significantly. Histopathologic scores were significantly better and the rate of apoptosis was lower in the 2-APB group. Conclusions 2-APB reduces oxidative stress and damage caused by renal I/R injury. The results of this study demonstrate that 2-APB can be used as an effective agent against I/R injury in the kidney. © 2014 Elsevier Inc. All rights reserved.