Antiproliferative properties of the lazaroids U-83836E and U-74389G on glioma cells in vitro
Pathology and Oncology Research, cilt.5, sa.3, ss.223-228, 1999 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 5 Sayı: 3
- Basım Tarihi: 1999
- Doi Numarası: 10.1053/paor.1999.0202
- Dergi Adı: Pathology and Oncology Research
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.223-228
- Anahtar Kelimeler: 21-aminosteroids, C6, Glioma, MTT, U-74389G, U-83836E
- Kütahya Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
The 21-aminosteroids (lazaroids) are a new family of steroid compounds that inhibit lipid peroxidation reactions. They are novel antioxidant agents, which have been shown to have antiproliferative properties on cancer cells and also are thought to prevent free radical-mediated blood-brain barrier damage. In order to understand the effect of lazaroids on glioma, we tested U-83836E and U-74389G at doses ranging between 0.1-100 μM on primary cultures of glioblastoma multiforme from three patients, rat C6 glioma cell line, and 5th subculture established from one of the patients. The effects of both compounds on cell proliferation were determined using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay. U- 83836E in the primary cultures was found to have 50% inhibitory concentrations (IC50) of 6.30, 6.75 and 6.50 μM, respectively. The IC50 value of U-74389G was calculated as 91 μM in only one of the patients. On C6 glioma cells, while the IC50 of U-83836E was 45 μM, U-74389G showed no cytotoxic effect. On the 5th subculture, U-83836E had an IC50 of 37.5 μM, but the cytotoxic effects of U-74389G was less than in that of the primary culture. In conclusion, these compounds were found to be more cytotoxic in primary culture than the cell lines and there were also differences between their members in the inhibition of cell survival.