Respirometric evaluation and modeling of the impact of continuous benzo[a]anthracene feeding on activated sludge
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, cilt.94, sa.8, ss.2621-2629, 2019 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 94 Sayı: 8
- Basım Tarihi: 2019
- Doi Numarası: 10.1002/jctb.6071
- Dergi Adı: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2621-2629
- Anahtar Kelimeler: benzo[a]anthracene, chronic impact, oxygen uptake rate, biodegradation kinetics, substrate binding, POLYCYCLIC AROMATIC-HYDROCARBONS, ACETATE UTILIZATION, SEWAGE SLUDGES, KINETICS, PEPTONE, BIODEGRADATION, STORAGE, EXTRACTION, PRINCIPLES, REMOVAL
- Kütahya Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
BACKGROUND This study evaluated the impact of continuous benzo[a]anthracene (B[a]A) feeding on the biodegradation of peptone/meat extract mixture by means of respirometric testing and modeling results, and microbial community analyses. A sequencing batch reactor (SBR) was operated at a sludge age of 10 days. At steady state, it was sustained with the peptone mixture feeding alone for 21 days. The following 21 days involved continuous dosing of 11 mg L-1 of B[a]A together with peptone mixture. In the last period of 7 days, B[a]A feeding was stopped. Storage of poly-hydroxy alkanoates (PHA) occurred during substrate utilization. A compatible model was calibrated using the oxygen uptake rate (OUR) and PHA profiles characterizing each phase. RESULTS Continuous exposure to B[a]A did not affect the microbial growth process, but slightly increased PHA storage rate and growth on stored PHA. Hydrolysis rates were increased at first exposure followed by a significant decrease. Substrate binding also occurred at first exposure and declined by the end of B[a]A feeding. A major change was observed in biomass composition due to continuous exposure to B[a]A. CONCLUSION The major novelty of the study was to prove that B[a]A altered the composition of the enriched microbial culture, characterized by slightly different process kinetics, especially for hydrolysable COD fractions. (c) 2019 Society of Chemical Industry