Evaluation of Lateral Hypothalamic Area Catecholamine Levels Following Intravenous Glucose Administration by Microdialysis Method in Rats

Solak H., Görmüş Z. I. S., Koca R. Ö.

Selçuk Tıp Dergisi, vol.38, no.1, pp.44-51, 2022 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 38 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.30733/std.2022.01545
  • Journal Name: Selçuk Tıp Dergisi
  • Journal Indexes: TR DİZİN (ULAKBİM), Index Copernicus
  • Page Numbers: pp.44-51
  • Kütahya Health Sciences University Affiliated: Yes


Aim: We aimed to investigate the effects of intravenous glucose administration in the lateral hypothalamic area (LHA) on the levels of noradrenaline, dopamine and their metabolites dihydroxyphenylglycol (DHPG)- dihydroxyphenylaceticacid (DOPAC) by brain microdialysis method. Materials and Methods: Adult male Wistar albino rats in 2 normally fed groups and 2 groups of restricted food intake for 24 hours (saline and glucose administered) were used. Microdialysis procedures were performed on the rats in LHA under anesthesia and samples were collected in 20 minutes. After the first samples were recorded as control, 0.9% saline was administered to the control groups and 50% glucose solution was administered intravenously to the glucose groups at a dose of 1.4 ml/kg. During the next 40 minutes, samples were collected and analyzed on the HPLC-ECD system. One-way ANOVA was used for statistical evaluation. Results: Noradrenaline concentration was higher in fasted rats than in satiated animals at baseline. Noradrenaline levels at the 20th minute were significantly decreased in both fasted-satiated groups compared to control group (p=0.01). There was no statistically significant difference in the 40th minute noradrenaline values and dopamine-DHPG-DOPAC levels compared to control. Conclusion: Systemic glucose administration decreased LHA noradrenaline concentration in fasted and satiated rats. It can be mentioned that noradrenergic neurotransmission in LHA can be modulated by plasma glucose.