The effect of N-acetylcysteine (NAC) on liver and renal tissue inducible nitric oxide synthase (iNOS) and tissue lipid peroxidation in obstructive jaundice stimulated by lipopolysaccharide (LPS)


Caglikulekci M., Pata C., Apa D., Dirlik M., Tamer L., Yaylak F. , ...Daha Fazla

PHARMACOLOGICAL RESEARCH, cilt.49, ss.227-238, 2004 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 49 Konu: 3
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.phrs.2003.09.013
  • Dergi Adı: PHARMACOLOGICAL RESEARCH
  • Sayfa Sayıları: ss.227-238

Özet

Morbidity and mortality rates are very high in obstructive jaundice when it is associated with sepsis and multiple organ failure. Nitric oxide (NO) formation and increased expression of inducible nitric oxide synthase (iNOS) also take place in obstructive jaundice (OJ). N-Acetylcysteine (NAC) has a beneficial effect by demonstrating anti-inflammatory activity such as inhibits cytokine expression/release, inhibiting the adhesion molecule expression and inhibiting nuclear factor kappa B (NFkappaB). The aim of this study was to investigate the effects of NAC on liver and renal tissue iNOS, and liver tissue lipid peroxidation in lipopolysaccharide (LPS) induced obstructive jaundice. We randomized 48 rats into six groups. Group A: Sham group; group B: OJ group; group C: OJ + NAC; group D: OJ + LPS (Escherichia coli LPS serotype L-2630, 100 mg, Sigma) group E: OJ + NAC + LPS; group F: OJ + LPS + NAC. NAC was started subcutaneously 100 mg/kg. LPS was injected intraperitoneally and then at the tenth day we sacrificed the rats.