The Protective Effects of Melatonin and Vitamin E against Renal Ischemia-Reperfusion Injury in Rats

Yalçın Ö.

RENAL FAILURE, no.ISSN: 0886-022X print / 1525-6049 online DOI: I 0. I 080/08860220'7 0 139 17 38, pp.535-542, 2007 (SCI-Expanded)

  • Publication Type: Article / Article
  • Publication Date: 2007
  • Journal Name: RENAL FAILURE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.535-542
  • Kütahya Health Sciences University Affiliated: No


Reactive oxygen species EOS) were shown to contribute to the çellular damage induced by ischemia_reperfusion. The purpose of this study was to investigate and compare the efficiency of melatonin and vitamin E in the reduction of injury induced by ROS in a rat model of renal isçhemia-reperfusion. Twenty-four Wistar-albino rats were divided into fow groups. Rats in the Sham group were given saline I ml/kg, intraperitoneally (ip) 72h,48 h, 24h, and 30 min before the sham operation. Rats in ischemia-reperfusion (IR), üt+Melatonin, and IR+Vitamin E groups were given saline (l mL/ kg), melatonin (10 mg&g), and vitamin E (100 mg&g) ip, respeatively, 72h,48 h, 24h, and 30 min before the isçhemia for 60 min, followed by reperfusion for 60 min. The blood samples and kidney tissues of the rats were taken under anesthesia. Ischemia-reperfusion significantly increased urea, creatinine, and malondialdehyde O4DA) levels, and decreased superoxide dismutase (SOD) and catalase (CAT) activities. Histopathological findings of the IR group confirmed that there was renal impairment by cast formation and tubular necrosis in the tubular epithelium. In the lR+Melatonin group, while MDA levels significantly decreased, SOD activities inçreased. In the lR+Melatonin grouP, the level of tubular neçroşis and çast formation are significantly decreased than those Seen in the ischemia-reperfusion group. Melatonin in particular was effective to reverse hot ischemia of kidney by its antioxidant effects. These results may indicate that melatonin prefreatment protects against functional, biochemical, and morphological damage better than vitamin E in renal ischemia-reperfusion injury.