TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.43, sa.5, ss.49-50, 2018 (SCI Expanded İndekslerine Giren Dergi)
OBJECTIVES: Renal ischemia/reperfusion (I/R) may cause distant tissue damage.
Boric acid (BA) with antioxidant properties is suggested to be a protective agent
against I/R injury. The aim of this study was to investigate the neuroprotective
effects of boric acid on brain against renal I/R induced oxidative damage.
MATERIALS and METHODS: 35 rats were divided into five groups: sham,
I/R, I/R+50mg/kg BA, I/R+100mg/kg BA and I/R+200mg/kg BA. Sham
group was only subjected to surgical stress procedure. In I/R group, left
renal artery was isolated and ischemia was induced for 45 minutes with
atraumatic vascular clamp, then followed by 24-hour reperfusion. In I/
R+BA groups, BA was administered intraperitoneally 10 minutes before
reperfusion, unlike the I/R group. In rat brain tissues MDA levels, GSH
levels, SOD activities and NO levels were measured spectrophotometrically.
RESULTS: Renal I/R caused a significant increase in MDA and NO levels as
compared to the values of the control group (P<0.01), but caused a decrease in GSH
levels and SOD activities.Conversely, rats treated with BA prior to reperfusion
showed a significant reduction in the brain MDA and NO levels as compared to
renal I/R group, on the other hand was an increase in GSH levels and SOD activities.
CONCLUSIONS: This study showed protective effects of BA on brain tissue
against renal I/R injury. BA treatment can decrease lipid peroxidation levels
and increase antioxidant enzymes activities in brain hippocampus of renal I/R
induced rats. Our results indicate that BA is useful for brain function maintaining
in renal I/R induced rats.
Keywords: Renal Ischemia/Reperfusion, Brain, Boric Acid, Oxidative Damage,