The dual role of boron in vitro neurotoxication of glioblastoma cells via SEMA3F / NRP2 and ferroptosis signaling pathways
ENVIRONMENTAL TOXICOLOGY AN INTERNATIONAL JOURNAL, cilt.38, sa.1, ss.70-77, 2023 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 38 Sayı: 1
- Basım Tarihi: 2023
- Doi Numarası: 10.1002/tox.23662
- Dergi Adı: ENVIRONMENTAL TOXICOLOGY AN INTERNATIONAL JOURNAL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, Greenfile, MEDLINE, Pollution Abstracts, Veterinary Science Database
- Sayfa Sayıları: ss.70-77
- Anahtar Kelimeler: boric acid, brain tumor, cancer, cytotoxicity, ferroptosis, Semaphorin
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Kütahya Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Glioblastoma multiform (GBM) is a malignant tumor cancer that originates from the
star-shaped glial support tissues, namely astrocytes, and it is associated with a poor
prognosis in the brain. The GBM has no cure, and chemotherapy, radiation therapy,
and immunotherapy are all ineffective. A certain dose of Boric acid (BA) has many
biochemical effects, conspicuously over antioxidant/oxidant rates. This article sought
to investigate the modifies of various doses of BA on the glioblastoma concerning
cytotoxicity, ferroptosis, apoptosis, and semaphorin–neuropilin signaling pathway.
The Cytotoxic activity and cell viability of BA (0.39–25 mM) in C6 cells were tested
at 24, 48, and 72 h using 3-(4,5-dimethylthiazol, 2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The IC50 concentration of BA at 1.56 mM was found and cell lysate used
for biochemical analysis. Glutathione peroxidase 4 (GPx4) and ACLS4 levels of ferroptosis, levels of total antioxidant (TAS) and oxidant (TAS) parameters, malondialdehyde
(MDA), apoptotic proteins as caspase 3 (CASP3) and caspase 7 (CASP7) were measured. The ferroptosis, semaphoring–neuropilin, apoptotic pathway markers and cell
counts were analyzed with flow cytometry, Q-PCR, Western and Elisa technique in
the C6 cell lysate. BA triggered ferroptosis in the C6 cells dose-dependently, affecting
the semaphorin pathway, so reducing proliferation with apoptotic compared with
untreated cell as control group (p < .05). This study revealed that BA, defined as trace
element and natural compound, incubated ferroptosis, total oxidant molecules, and
caspase protein in a dose-dependently by disrupting SEMA3F in tumor cells