Comparison of pre-treatment and post-treatment use of selenium in retinal ischemia reperfusion injury
International Journal of Ophthalmology, cilt.8, sa.2, ss.263-268, 2015 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 8 Sayı: 2
- Basım Tarihi: 2015
- Doi Numarası: 10.3980/j.issn.2222-3959.2015.02.09
- Dergi Adı: International Journal of Ophthalmology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.263-268
- Anahtar Kelimeler: Apoptosis, Ischemia-reperfusion, Oxidative stress, Post-treatment, Pre-treatment, Retina, Selenium
- Kütahya Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
AIM: To investigate the effects of selenium in rat retinal ischemia reperfusion (IR) model and compare pretreatment and post-treatment use. METHODS: Selenium pre-treatment group (n=8) was treated with intraperitoneal (i.p.) selenium 0.5 mg/kg for 7d and terminated 24h after the IR injury. Selenium posttreatment group (n=8) was treated with i.p. selenium 0.5 mg/kg for 7d after the IR injury with termination at the end of the 7d period. Sham group (n=8) received i.p. saline injections identical to the selenium volume for 7d with termination 24h after the IR injury. Control group (n=8) received no intervention. Main outcome measures were retina superoxide dismutase (SOD), glutathione (GSH), total antioxidant status (TAS), malondialdehyde (MDA), DNA fragmentation levels, and immunohistological apoptosis evaluation. RESULTS: Compared to the Sham group, selenium pre -treatment had a statistical difference in all parameters except SOD. Post -treatment selenium also resulted in statistical differences in all parameters except the MDA levels. When comparing selenium groups, the pre-treatment selenium group had a statistically higher success in reduction of markers of cell damage such as MDA and DNA fragmentation. In contrast, the post - selenium treatment group had resulted in statistically higher levels of GSH. Histologically both selenium groups succeeded to limit retinal thickening and apoptosis. Pre -treatment use was statistically more successful in decreasing apoptosis in ganglion cell layer compared to post-treatment use. CONCLUSION: Selenium was successful in retinal protection in IR injuries. Pre -treatment efficacy was superior in terms of prevention of tissue damage and apoptosis.