ABS TRACT Objective: Traditional and complementary medicine (T&CM) practices are ancient methods that support modern methods and contribute to protecting and improving people’s health. The study aimed to determine university students’ knowledge, attitudes and behaviours about T&CM practices and their determinants. Material and Methods: The study was cross-sectional. Research data were collected between 01.11.2022/31.12.2022. The study population consists of undergraduate students studying at Alanya Alaaddin Keykubat University in the 2021-2022 academic year (n=11,975). It was aimed to reach 760 students in total. Data were collected in three forms: A socio-demographic questionnaire form, a knowledge and experience form about T&CM applications, and a T&CM Attitude Scale. Results: The mean age of the participants was 20.98±1.76 years (minimum: 18-maximum: 29), and 57.9% (n=440) were over 20 years old. 62% (n=471) of the participants were female. Of the research group, 48.8% (n=371) stated that they had no knowledge about T&CM applications, 42.5% (n=323) stated that they had some knowledge, and 8.7% (n=66) stated that they knew in detail. A significant difference was found between the total score of the attitude scale towards using complementary therapies and the participants’ department, class, smoking status and T&CM experience (p<0.05). Conclusion: Knowledge and experience in T&CM applications positively affect the attitude towards T&CM applications.