Screening of thermotolerant microfungi isolated from hot spring for production of extracellular enzymes


Berikten D. , Kıvanç M.

Current Opinion In Biotechnology, cilt.22, ss.90, 2011 (SCI Expanded İndekslerine Giren Dergi)

  • Cilt numarası: 22 Konu: 1
  • Basım Tarihi: 2011
  • Dergi Adı: Current Opinion In Biotechnology
  • Sayfa Sayıları: ss.90

Özet

The purpose of this study was to collect and isolate filamentous fungi from hot spring water of different regions of Kütahya province and screening of microorganisms with potential to produce lipase, amylase, cellulase, phytase, protease. We were carried the isolation of filamentous fungi from hot water samples by using the membrane filtration method at 45°C. Isolates were tested for temperature. Macroscopic and microscopic properties of fungi isolates is examined and they were identified microscopically to genus and species level with traditional methods using diagnostic literatures. And carbohydrate tests of the isolates were done by biolog microstation system. The screening of fungi that produce extracellular lipase, amylase, cellulase, phytase, protease was carried out in Petri dishes or tubes using specific mediums for different enzymes. Fifteen microfungi isolates were obtained from five different water samples. Applied temperature tests were revealed that these molds are thermotolerant. The fungi were identified as Aspergillus terreusRhizomucor pussillusMyriococcum albomycesAspergillus fumigatusAspergillus flavusHumicola sp. After the screening for enzyme production, it determined that all isolates produce lipase, all isolates except Humicola sp. produce amylase, A. terreusA. fumigatusRhizomucor pussillusHumicola sp. produce phytase and some of them produce cellulase, protease.