STACHYS SUBNUDA} EKSTRELERİNİN MEME KANSERİNE ANTİKANSER ETKİSİ
6th International and 35th National Biophysics Congress, Kayseri, Türkiye, 4 - 07 Eylül 2024, ss.197-198, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Kayseri
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.197-198
- Kütahya Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim: Stachys subnuda (SS) is a species belonging to the Stachys genus within the Lamiaceae
family. It is known that species of the Stachys genus are rich in flavonoids, and their antioxidant properties have been demonstrated in previous studies.
Additionally, some species
have shown cytotoxic and
apoptotic effects on breast cancer cell lines. However, studies on the SS
species are quite limited. SS is endemic to Turkey and is commonly known as
"mountain tea" among the local population. Traditionally, SS has been used for skin, stomach, respiratory, and kidney ailments.
Methods: The aerial parts of the SS
plant were collected from Tunceli. The methanol extract of SS (SSM) and its
hexane (SSH), chloroform (SSC), ethyl acetate (SSEA), and aqueous methanol
(SSAM) fractions were prepared at the Faculty of Pharmacy, Marmara University.
The extracts were evaluated for cytotoxicity and apoptosis with CCK-8 and JC-1 mitochondrial membrane potential tests. GraphPad Prism
(version 8.0.1) was used for statistical analysis. One- way ANOVA followed by Tukey's test was performed.
Results: When SSM, SSH, SSC, SSEA, and
SSAM extracts were applied to MCF-7 breast cancer cells at various
concentrations (0, 10, 25, 50, 75, 100 µg/mL) for 24 hours, the IC50
values were
calculated as 30.45, 16.44, 77.73, 70.07, and 34.18 µg/mL, respectively. The
selected SSH extract statistically significantly depolarized the mitochondrial
membrane potential (p < 0.05).
Conclusions: The cytotoxic and apoptotic effects
of the SS species on the MCF-7
cell line were investigated for the first time,
suggesting that SSH may have therapeutic potential.