Sıçan böbrek dokusunda etanolün akut toksisitesi ve borik asitin koruyucu rolü


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Çalışkan A., Güney T. G. , Kar F. , Çakır Gündoğdu A. , Özbayer C.

Biological Diversity and Conservation, vol.15, no.1, pp.107-113, 2022 (Refereed Journals of Other Institutions)

  • Publication Type: Article / Article
  • Volume: 15 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.46309/biodicon.2022.1084827
  • Title of Journal : Biological Diversity and Conservation
  • Page Numbers: pp.107-113

Abstract

In this study, the potential protective effect of boric acid in the process of inducing oxidative stress on kidney

tissue due to acute alcohol toxicity was evaluated by biochemical and histological examination methods. In the study,

250-300 gr. Wistar albino female rats aged 3-4 months, matched in weight, age and weight, were used. 28 female rats

were used, seven animals in each group: control, ethanol, boric acid (BA) and ethanol + BA.Renal tissue samples were

taken for histology and biochemistry. Kidney damage was assessed using hematoxylin and eosin (H&E) staining. At the

same time, total antioxidant level (TAS), total oxidant level (TOS) and oxidative stress index (OSI) were measured to

evaluate oxidative stress as biochemical markers. While TOS and OSI levels were significantly lower in the BA group

compared to the ethanol group (p<0.05), no statistically significant change was observed in the TAS level. In the

ethanol-treated group, deterioration in tubule morphology occurred as a result of epithelial cells spilling into the lumen

in H&E staining. In the boric acid administered group, the structure of the renal cortex was observed to be normal,

similar to the control group.In the boric acid+ethanol group, dilatation was observed to be less than in the ethanol

administered group with normal renal tubules. As acute ethanol toxicity increases, lipid peroxidation and oxidative

stress increase. BA administration has been observed to reduce acute ethanol toxicity oxidative stress-induced kidney

damage, as demonstrated by both biochemical parameters and histological results.

Key words: Boric acid, Acute ethanol consumption, Kidney, Oxidative stress