Background Acne vulgaris is a common disease affecting adolescents. There is not comprehensive data on acne prevalence in the Central Anatolia Region in particular. Etiology of acne is not clarified yet. Acne might be related to environmental factors. There is increasing evidence supporting acne and diet relationship. Objectives The aim of the study was to determine the acne prevalence in adolescents in the city of Eskisehir, located in the Central Anatolia, Turkey in addition to evaluate factors affecting acne and its relationship with dietary habits. Methods A cross-sectional study was conducted on 2300 participants aged 13-18 years. The participants were asked to complete a questionnaire form consisting information about acne and a questionnaire form consisting information about dietary habits (The Adolescent Food Habits Checklist). In addition an objective evaluation of acne was determined. Results The mean age of students with acne was 15.10 +/- 1.53. The current acne prevalence was 60.7%. Although 21% of the participants had severe acne (grade 34) and 25% developed sequelaes, only 11.5% of all participants consulted a doctor. The participants without acne had healthier dietary habits than participants with acne (P < 0.05). Frequent fat intake (OR = 1.39, 95% CI: 1.061.82), frequent sugar intake (OR = 1.30, 95% CI: 1.051.60), frequent eating sausages, burgers (OR = 1.24, 95% CI: 1.031.48), frequent eating pastries, cakes (OR = 1.20, 95% CI: 1.011.43) were associated with increased risk for acne. Conclusions Acne prevalence is high among adolescents in Eskisehir but the rate of consulting doctor is low. Increasing public awareness is critical for convincing adolescents to seek medical help earlier. Acne was related with dietary habits. Fat, sugar and fast food consumption is found to be positively correlated with acne prevalence.