This study was to evaluate the effect of long-term exercise training on blood fasting glucose (BFG) level and weight in alloxan administered mice. Forty mice, Mus musculus species was alloxan administered by an intraperitoneal (i.p.) injection 3 times in two weeks intervals (200 mg/kg b.w.), and training program was administered by treadmill for 10 min 5 day/week and exercise period was prolonged for 2.5 min in a week. In the 8th week it was observed that sedentary male group BFG levels (172.3 mg/dl) were statistically significant higher than other groups (p < 0.05). Measurements at the end of the 10th week were showed that untrained sedentary group's BFG were found higher with statistically significant levels than trained groups (p < 0.05). Sedentary male group weight values were observed to be higher than other groups (p < 0.05). Long-term physical training has protective effects against chemically induced diabetes in mice.