Is a single nucleotide polymorphism a risk factor for lung cancer in the matrix metalloproteinase-2 promoter?


BAYRAMOĞLU A. , GÜNEŞ H. V. , METİNTAŞ M., DEĞİRMENCİ İ. , AKIN A., KURT H.

MOLECULAR BIOLOGY REPORTS, vol.38, no.3, pp.1469-1474, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 3
  • Publication Date: 2011
  • Doi Number: 10.1007/s11033-010-0253-5
  • Title of Journal : MOLECULAR BIOLOGY REPORTS
  • Page Numbers: pp.1469-1474
  • Keywords: Lung cancer, MMP-2 gene C1306T polymorphism, Plasma MMP-2 enzyme activity, FUNCTIONAL HAPLOTYPES, GENETIC POLYMORPHISMS, COLORECTAL-CANCER, MMP-2, METASTASIS, MATRIX-METALLOPROTEINASE-2, INHIBITORS, EXPRESSION, DISEASE

Abstract

We aimed to investigate the association of polymorphism frequencies of MMP-2 C1306T and MMP-2 plasma enzyme activity in lung cancer patients. In this study 300 genomic DNA (200 lung cancer patients + 100 no lung cancer) were analyzed. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism (RFLP) and electrophoresis. Plasma MMP-2 enzyme activity levels were measured by using ELISA. Sex, asbestos expose and smoking might be risk factors for lung cancer. The frequencies of C1306T genotypes in controls CC 65%, CT 32%, TT 3% and in patients CC 61%, CT 37%, TT2 % were found. It was determined that CC genotype frequency increase significantly in patients and controls. Plasma MMP-2 enzyme activity levels were increased in lung cancer patients according to controls. Finally, we claimed that determining of MMP-2 enzyme level can be used as a marker in lung cancer.