Is a single nucleotide polymorphism a risk factor for lung cancer in the matrix metalloproteinase-2 promoter?
MOLECULAR BIOLOGY REPORTS, cilt.38, sa.3, ss.1469-1474, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 38 Sayı: 3
- Basım Tarihi: 2011
- Doi Numarası: 10.1007/s11033-010-0253-5
- Dergi Adı: MOLECULAR BIOLOGY REPORTS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1469-1474
- Anahtar Kelimeler: Lung cancer, MMP-2 gene C1306T polymorphism, Plasma MMP-2 enzyme activity, FUNCTIONAL HAPLOTYPES, GENETIC POLYMORPHISMS, COLORECTAL-CANCER, MMP-2, METASTASIS, MATRIX-METALLOPROTEINASE-2, INHIBITORS, EXPRESSION, DISEASE
- Kütahya Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
We aimed to investigate the association of polymorphism frequencies of MMP-2 C1306T and MMP-2 plasma enzyme activity in lung cancer patients. In this study 300 genomic DNA (200 lung cancer patients + 100 no lung cancer) were analyzed. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism (RFLP) and electrophoresis. Plasma MMP-2 enzyme activity levels were measured by using ELISA. Sex, asbestos expose and smoking might be risk factors for lung cancer. The frequencies of C1306T genotypes in controls CC 65%, CT 32%, TT 3% and in patients CC 61%, CT 37%, TT2 % were found. It was determined that CC genotype frequency increase significantly in patients and controls. Plasma MMP-2 enzyme activity levels were increased in lung cancer patients according to controls. Finally, we claimed that determining of MMP-2 enzyme level can be used as a marker in lung cancer.