Antistaphylococcal activity of DX-619, a new des-F(6)-quinolone, compared to those of other agents


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Bogdanovich T., Esel D. , Kelly L., Bozdogan B., Credito K., Lin G., et al.

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, cilt.49, ss.3325-3333, 2005 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 49 Konu: 8
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1128/aac.49.8.3325-3333.2005
  • Dergi Adı: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
  • Sayfa Sayısı: ss.3325-3333

Özet

The in vitro activity of DX-619, a new des-F(6)-quinolone, was tested against staphylococci and compared to those of other antimicrobials. DX-619 had the lowest MIC ranges/MIC(50)s/MIOC(90)s (mu g/ml) against 131 Staphylococcus aureus strains (<= 50.002 to 2.0/0.06/0.5) and 128 coagulase-negative staphylococci (0.004 to 0.25/0.016/ 0.125). Among strains tested, 76 S. aureus strains and 51 coagulase-negative staphylococci were resistant to ciprofloxacin. DX-619 had the lowest MIC50/MIC90 values against 127 quinolone-resistant staphylococci (0.125/0.5), followed by sitafloxacin (0.514), moxifloxacin (2/8), gatifloxacin (4/16), levofloxacin (16/> 32), and ciprofloxacin (> 32 > 32). Raised quinolone MICs were associated with mutations in GyrA (S84L) and single or double mutations in GrlA (S80F or Y; E84K, G, or V) in all S. aureus strains tested. A recent vancomycin-resistant S. aureus (VRSA) strain (Hershey) was resistant to available quinolones and was inhibited by DX-619 at 0.25 mu g/ml and sitafloxacin at 1.0 mu g/ml. Vancomycin (except VRSA), linezolid, ranbezolid, tigecycline, and quinupristin-dalfopristin were active against all strains, and teicoplanin was active against S. aureus but less active against coagulase-negative staphylococci. DX-619 produced resistant mutants with MICs of 1 to > 32 mu g/ml after < 50 days of selection compared to 16 to > 32 mu g/ml for ciprofloxacin, sitafloxacin, moxifloxacin, and gatifloxacin. DX-619 and sitafloxacin were also more active than other tested drugs against selected mutants and had the lowest mutation frequencies in single-step resistance selection. DX-619 and sitaffoxacin were bactericidal against six quinolone-resistant (including the VRSA) and seven quinolone-susceptible strains tested, whereas gatifloxacin, moxifloxacin, levofloxacin, and ciprofloxacin were bactericidal against 11, 10, 7, and 5 strains at 4 X MIC after 24 h, respectively. DX-619 was also bactericidal against one other VRSA strain, five vancomycin-intermediate S. aureus strains, and four vancomycin-intermediate coagulase-negative staphylococci. Linezolid, ranbezolid, and tigecycline were bacteriostatic and quinupristin-dalfopristin, teicoplanin, and vancomycin were bactericidal against two, eight, and nine strains, and daptomycin and oritavancin were rapidly bactericidal against all strains, including the VRSA. DX-619 has potent in vitro activity against staphylococci, including methicillin-, ciprofloxacin-, and vancomycin-resistant strains.