Research Information System
Medical Science and Discovery, vol.5, no.5, pp.192-197, 2018 (Peer-Reviewed Journal)
Objective:Fluoride increases the
production of reactive
oxygen and nitrogen
derivatives, causing oxidative
stress and cellular damage. Betaine,
an antioxidant and
an important methyl
donor, has been
reported to have potential protective
effects on cardiovascular and
neurodegenerative diseases in
recent years. In
this study, we
aimed to investigate
the neuroprotective effects
of betaine treatment against cellular damage caused by fluoride exposure
on rat synaptosomes.Material and Methods:In the experimental period,8 Wistar albino rats were decapitated and then
their frontal cortices removed and
divided into 4
equal cuts (total-32
cuts). Subsequently, an
appropriate experimental procedure was
applied to obtain
the synaptosomal fractions. The
determined doses of sodium fluoride
(NaF) and betaine were administered in
vitro at 37°C for 30 min. Synaptosomal glutathione
(GSH), malondialdehyde (MDA)
and nitricoxide (NO)
levels, and also
catalase (CAT) and
Ca+2/Mg+2 ATPase activities were measured spectrophotometrically.Results:According to
our results, NaF exposure caused a decrease in GSH levels, CAT and Ca+2/Mg+2
ATPase activities, and also an
increase in MDA
and NO levels,
significantly. MDA levels,
NO levels and
CAT activities were
closed to the
control group depends on the
betain doses. The best recovery in terms of synaptosomal GSH levels and
Ca+2/Mg+2 ATPase activities was found at 0.5 mM betaine concentration (P
<0.001).Conclusion:Our results showed
that betaine could
be a potential
neuroprotective therapeutic agent
against synaptosomal fluoride toxicity.Keywords:Fluoride,
Betaine, Synaptosomes, Neurodegeneration, Oxidative stress