Rat brain synaptosomes: In vitro neuroprotective effects of betaineagainst fluoride toxicity

Hacıoğlu C., Kar F., Kanbak G.

Medical Science and Discovery, vol.5, no.5, pp.192-197, 2018 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 5 Issue: 5
  • Publication Date: 2018
  • Doi Number: 10.17546/msd.421851
  • Journal Name: Medical Science and Discovery
  • Journal Indexes: Index Copernicus
  • Page Numbers: pp.192-197
  • Kütahya Health Sciences University Affiliated: No


Objective:Fluoride  increases  the  production  of  reactive  oxygen  and  nitrogen  derivatives,  causing  oxidative  stress  and  cellular damage.  Betaine,  an  antioxidant  and  an  important  methyl  donor,  has  been  reported  to  have  potential  protective  effects  on cardiovascular  and  neurodegenerative  diseases  in  recent  years.  In  this  study,  we  aimed  to  investigate  the  neuroprotective  effects  of betaine treatment against cellular damage caused by fluoride exposure on rat synaptosomes.Material and Methods:In the experimental period,8 Wistar albino rats were decapitated and then their frontal cortices removed and  divided  into  4  equal  cuts  (total-32  cuts).  Subsequently,  an  appropriate  experimental  procedure  was  applied  to  obtain  the synaptosomal  fractions. The determined doses of sodium  fluoride (NaF) and betaine were  administered in vitro at 37°C  for 30  min. Synaptosomal  glutathione  (GSH),  malondialdehyde  (MDA)  and  nitricoxide  (NO)  levels,  and  also  catalase  (CAT)  and  Ca+2/Mg+2 ATPase activities were measured spectrophotometrically.Results:According to our results, NaF exposure caused a decrease in GSH levels, CAT and Ca+2/Mg+2 ATPase activities, and also an  increase  in  MDA  and  NO  levels,  significantly.  MDA  levels,  NO  levels  and  CAT  activities  were  closed  to  the  control  group depends on the betain doses. The best recovery in terms of synaptosomal GSH levels and Ca+2/Mg+2 ATPase activities was found at 0.5 mM betaine concentration (P <0.001).Conclusion:Our  results  showed  that  betaine  could  be  a  potential  neuroprotective  therapeutic  agent  against  synaptosomal  fluoride toxicity.Keywords:Fluoride, Betaine, Synaptosomes, Neurodegeneration, Oxidative stress