Evaluation of cisplatin neurotoxicity in cultured rat dorsal root ganglia via cytosolic calcium accumulation

Erol K., YİĞİTASLAN S., Ünel Ç., KAYGISIZ B., YILDIRIM E.

Balkan Medical Journal, vol.33, no.2, pp.144-151, 2016 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 2
  • Publication Date: 2016
  • Doi Number: 10.5152/balkanmedj.2016.161110
  • Journal Name: Balkan Medical Journal
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.144-151
  • Keywords: Calcium, Cisplatin, Dorsal root ganglia, Primary cell culture
  • Kütahya Health Sciences University Affiliated: No

Abstract

Background: Calcium homeostasis is considered to be important in antineoplastic as well as in neurotoxic adverse effects of cisplatin. Aims: This study aimed to investigate the role of Ca2+ in cisplatin neurotoxicity in cultured rat dorsal root ganglia (DRG) cells. Study Design: Cell culture study. Methods: DRG cells prepared from 1-day old Sprague-Dawley rats were used to determine the role of Ca2+ in the cisplatin (10-600 μM) neurotoxicity. The cells were incubated with cisplatin plus nimodipine (1-3 μM), dizocilpine (MK-801) (1-3 μM) or thapsigargin (100-300 nM). Toxicity of cisplatinon DRG cells was determined by the MTT assay. Results: The neurotoxicity of cisplatin was significant when used in high concentrations (100-600 μM). Nimodipine (1 μM) but not MK-801 or thapsigargin prevented the neurotoxic effects of 200 μM of cisplatin. Conclusion: Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.