Alpha lipoic acid attenuates iron induced oxidative acute kidney injury in rats.

Cavdar, Z; Oktan, Ma; Ural, C; Kocak, AYŞE; Calisir, M; Heybeli, C; Yildiz, S; Ozbal, S; Arslan, S; Ergur, Bu; Yilmaz, O; Cavdar, C

doi:10.1080/10520295.2020.1812001

Alpha lipoic acid attenuates iron induced oxidative acute kidney injury in rats.

Cavdar Z., Oktan M., Ural C., Kocak A., Calisir M., Heybeli C., ...Daha Fazla

Biotechnic & histochemistry : official publication of the Biological Stain Commission, cilt.96, sa.6, ss.409-417, 2021 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 96 Sayı: 6
Basım Tarihi: 2021
Doi Numarası: 10.1080/10520295.2020.1812001
Dergi Adı: Biotechnic & histochemistry : official publication of the Biological Stain Commission
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
Sayfa Sayıları: ss.409-417
Anahtar Kelimeler: Alpha lipoic acid, iron, kidney injury, NOX4, PI3K, Akt, p38 MAPK, rat, TRACE-ELEMENTS, RENAL-FAILURE, STRESS, ACTIVATION, CELL, INHIBITION, APOPTOSIS, TISSUE
Kütahya Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

Iron has been implicated in oxidative tissue injury owing to its ability to generate reactive oxygen species (ROS). We investigated the reno-protective effects of alpha lipoic acid (ALA) by investigating its effects on the kidney isoform of NADPH oxidase (Nox4) and the specific signaling pathways, p38 MAPK and PI3K/Akt, which participate in apoptosis and survival, respectively. We established four groups of seven rats: control, 100 mg/kg ALA, 80 mg/kg iron sucrose (IS) and IS + ALA. IS and ALA were injected intravenously and rats were sacrificied after 6 h. The mRNA expression of the subunits of NADPH oxidase, Nox4 and p22phox; tumor necrosis factor-alpha (TNF-α); and kidney injury molecule-1 (KIM-1) were measured using quantitative real time polymerase chain reaction (qRT-PCR). Active caspase-3 protein expression was evaluated by immunostaining. Also, p38 MAPK and PI3K/Akt signaling pathways were analyzed using western blot. ALA suppressed the mRNA expression of Nox4, p22phox, TNF-α and KIM-1. Active caspase-3 protein expression induced by IS was decreased by ALA. ALA also suppressed p38 MAPK and activated the PI3K/Akt signaling pathway following IS administration. We found that ALA may be an effective strategy for preventing oxidative acute kidney injury caused by IS.