CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, no.35 (1), pp.50-54, 2008 (SCI-Expanded)
1. Testicular ischaemia-reperfusion injury is commonly seen in childhood. Inferülİty occurs in 25% of patients after unilateral testicular ischaemİa. It is has been reported that methylene blue has a positive effect in the reparation of ischaemia-reperfusion injury in different tissues. Therefore, we hypothesized that methylene blue may prevent the hazardous effects of İschaemia-reperfusion injury İn testİcular tissue after unilateral testicular torsion.
2. Thirty-two prepubertal Wistar-albino rats were divided into four groups. Testicular torsion was created by rotating the right testis 720ᵒ in a clockwise direction for 5 h in all groups except for Group C, which was the sham control group. In GroupT, bilateraı orchiectomy was performed following the torsion period. In Group TD, both testes were removed 5 days after the torsion period. In Group MB, methylene blue (1 mg/kg, i.p.) was administered 40 min before detorsion and once daily over 5 days; then, both testes were harvested. Tİssue levels of malondialdehyde (MDA)' serum levels of creatine kİnase (CK), mean testicular biopsy score (MTBS) and mean seminifer tubule diameter (MSTD) were determİned.
3. There was a significant difference in MTBS between Groups T and TD (P < 0.05) in both ipsilateral and contralateral testes. In the contralateral testis, treatment with methylene blue decreased MTBS and MSTD (P < 0.05) and increased MDA levels (P < 0.05). In Group T, mean serum CK concentrations were higher than İn any of the other groups (P < 0.05).
4. After 5 h of unilateral testicular torsİon and a 5 day reperfusion perİod, serious tissue damage occurred on both the ipsilateral and contralateral sides. Serum CK concentratİons may be an indicator for İschaemia, but not for ischaemia-reperfusion injury. Contrary to our hypothesis, methylene blue increased contralateral testicular damage after unilateral testicular torsion and exacerbated oxidative events.