Lack of Association Between Type 2 Diabetes and the 3673G/A and 9041G/A Gene Variants of Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1)


Ozbayer C. , Kurt H., Kebapci M. N. , Cosan D. T. , Colak E., Gunes H. V. , ...Daha Fazla

INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH, cilt.86, ss.133-139, 2016 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 86
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1024/0300-9831/a000302
  • Dergi Adı: INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH
  • Sayfa Sayıları: ss.133-139

Özet

The vitamin K epoxide reductase complex subunit 1 (VKORC1) gene is expressed in many tissue types, and encodes the VKORC1 protein, which is a key enzyme in the vitamin K cycle. Although researchers have focused on the effects of vitamin K on glucose metabolism, and on its role in the development of type 2 diabetes (T2DM), no consensus has yet been reached. Therefore, here we aimed to investigate the association between VKORC1 variants and the risk of T2DM. The 3673G/A (rs9923231) and 9041G/A (rs7294) VKORC1 variants were investigated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 100 control individuals and 100 patients with T2DM. The genomic regions were amplified by PCR; amplicons were digested using the AciI and NciI enzymes and visualized by agarose gel electrophoresis. The genotype frequencies of the 3673G/A variants were GG (22%), GA (56%), and AA (22%) in the control group and GG (19%), GA (52%), and AA (29%) in patients with T2DM (p > 0.05). The genotype frequencies of the 9041G/A variants were GG (37%), GA (53%), and AA (10%) in the control group and GG (46%), GA (45%), and AA (9%) in patients with T2DM (p > 0.05). In conclusion, we found no significant correlation between the control group and patients with T2DM, with regard to the different genetic models of the 3673G/A and 9041G/A variants. These data suggest that these VKORC1 gene variants may not be linked to T2DM.