The effects of ethanol on glucose 6-phosphate dehydrogenase enzyme activity from human erythrocytes in vitro and rat erythrocytes in vivo


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Büyükokuroğlu M. E. , Altıkat S. , Çiftci M.

ALCOHOL AND ALCOHOLISM, cilt.37, ss.327-329, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 37 Konu: 4
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1093/alcalc/37.4.327
  • Dergi Adı: ALCOHOL AND ALCOHOLISM
  • Sayfa Sayıları: ss.327-329

Özet

Aims: The effects of ethanol on erythrocyte glucose 6-phosphate dehydrogenase (G6PD) activity were investigated under in vitro and in vivo conditions. Methods: For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme activity was determined spectrophotometrically by the Beutler method. Results: The in vitro study showed that the I-50 value was 17 mM for ethanol. In the case of the in vivo study, a 2 ml/kg dose of ethanol significantly inhibited the G6PD activity. The inhibition rate after ethanol administration was 59%, 40% and 6% at 1, 3 and 6 h after, respectively. Conclusions: The results of this study suggest that ethanol has a significant inhibitory effect on the G6PD activity both in vivo and in vitro.