Research Information System
29th BASS Congress, Belgrade, Serbia, 24 - 26 April 2025, pp.206, (Summary Text)
Objectives: The elimination of microorganisms is critical for the success of regenerative endodontic
treatments. This study aims to evaluate the antibacterial efficacy of calcium hydroxide (Ca(OH)₂),
triple antibiotic paste (TAP), curcumin, riboflavin, sodium hypochlorite (NaOCl), and saline solution
as intracanal medicaments against Enterococcus faecalis in the root dentin of mature teeth for
regenerative treatments using culture methods and real-time Polymerase Chain Reaction (PCR).
Methods: Sixty extracted single-rooted maxillary central incisors were decoronated to prepare 8
mm-long dentin cylinders.The root canals were inoculated with E. faecalis ATCC 29212 and incubated
for 21 days. The samples were randomly divided into six groups (n=10) based on the medicaments
applied. The medicaments were administered for a duration of 14 days. Antibacterial efficacy was
assessed using samples collected from dentin cylinders with paper points and analyzed via culture
methods and real-time PCR. Statistical analyses were performed using SPSS software, employing
ANOVA and Duncan tests (p<0.05).
Results: TAP and curcumin exhibited the highest antibacterial efficacy in both methods (p<0.001),
while NaOCl and Ca(OH)₂ showed moderate effectiveness. Riboflavin and saline demonstrated the
lowest efficacy (p<0.001). None of the medicaments completely eliminated Enterococcus faecalis
from the root canals.
Conclusions: TAP and curcumin are the most effective medicaments for the treatment of mature
teeth. Curcumin, as a natural and biocompatible agent, holds promise for regenerative endodontics
and may serve as a potential alternative to synthetic agents.
Keywords: Regenerative Endodontic Treatment, Enterococcus Faecalis, Calcium Hydroxide, Triple
Antibiotic Paste, Curcumin